International Investigative Dermatology 2018 Annual Meeting

Every five years, the European Society for Dermatological Research, the Japanese Society of Investigative Dermatology, and the Society of Investigative Dermatology 召开一个联合会议,承认来自这些协会的所有科学家在一个地方开会的科学价值的提高. Dr. Michael Bachelor将展示一种新型特应性皮炎皮肤模型的发展的海报,该模型模拟皮肤状况 eczema and provides a platform for disease research and treatment methods. Also being presented is research done for NASA which assessed the effects of ammonia vapor exposure on human skin. The International Space Station utilizes ammonia in its coolant system, 这项研究评估了氨渗透到飞船上的生活空间时对宇航员皮肤的影响.

Stop by and see us at Booth #206


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Induction of an Atopic Dermatitis-Like Phenotype in a Full-Thickness in vitro Human Skin Model | ID: LB1583

Jennifer Molignano, Kaitlyn Marengo, Jonathan Oldach, Gina Stolper, Max Li and Michael Bachelor

im体育app Corporation, Ashland, MA

Thursday, May 17, 11:45 am – 1:45 pm

特应性皮炎(AD)是一种以角质细胞分化缺陷和皮肤屏障功能障碍为特征的最常见的皮肤炎症性疾病.  Despite its prevalence, an in vitro, commercial model of AD is not currently available to facilitate basic research and therapeutic development.  Using the reconstructed full thickness human epidermal model (EpiDermFT), 一种由Th2细胞因子组成的炎症鸡尾酒被用来诱导模仿特应性皮炎表型的变化.  Following treatment with IL-4, IL-13 and IL-31, significant changes in several markers of differentiation and skin barrier integrity were observed, closely resembling AD skin.  组织学分析显示治疗与il - 4, IL-13, and IL-31 reduced the presence of the stratum granulosum and induced spongiosis in the epidermis.  Upregulation of pro-inflammatory mediators, such as IL-8, and down regulation of differentiation markers (keratin 1, keratin 10, involucrin, filaggrin) were observed at both the gene and protein level. 这些发现表明,用Th2细胞因子治疗表皮mft组织模型诱导了一种与AD特征一致的表型, including altered differentiation and barrier function.  我们预期这里描述的模型将有助于筛选和评估治疗特应性皮炎的有效成分和其他分子.

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Effects of Ammonia Vapor Exposure on Viability in a Human Skin Tissue Model | Abstract ID: 431729 Final ID: 716

Michael Bachelor1, Patrick Hayden1, Jennifer Molignano1, Hector D Garcia2, Katherine Toon3, Valerie E. Ryder3

1im体育app Corporation, 2KBRWyle and 3NASA Johnson Space Center

Thursday, May 17, 11:45 am – 1:45 pm

In this study, EpiDermFT, an in vitro 3D model of human skin, was used to evaluate the effects of ammonia vapor on skin to simulate in vivo exposure.  国际空间站(ISS)的美国部分在主动热控制系统的外部循环中使用氨. 有一个很低的可能性的故障场景将允许氨通过界面热交换器进入国际空间站的可居住体积. Protective masks have been developed to protect the eyes and respiratory tracts of crew in the event of a breach, 但氨气浓度升高对完整人体皮肤影响的剂量/反应在很大程度上是未知的.  Since EpiDermFT is cultured at the air-liquid interface and is amenable to topical exposures of test material, this 3D tissue model was an appropriate test system to simulate in vivo exposure to ammonia vapor.  vitcellell开发了一种定制的暴露装置,将表皮mft组织的根尖表面暴露在清洁空气(作为对照)或浓度为10的氨气中, 100, 1000, 10,000, 30,000 or 50,000 ppm for 1, 5 or 20 minutes. 在暴露后立即或暴露24小时后,使用MTT试验评估组织的细胞活力和表明毒性的形态学变化.  数据表明,浓度在10- 1000ppm之间的氨持续20分钟对皮肤组织活力没有立即或延迟(24小时)的影响. Signs of toxicity, especially 24 hr post-exposure were noted at 10,000 ppm ammonia and increased in severity with both increasing exposure time and concentration.  To our knowledge, this report represents the first evaluation of ammonia vapor on skin.  Altogether, 我们展示了表皮mft模型作为人类皮肤的相关替代品,用于评估对环境损害的细胞毒性的实用性.

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在包含功能性黑色素细胞的三维表皮模型中使用色度计测量皮肤色素沉着

Michael Bachelor, Bridget Breyfogle and Mitchell Klausner

im体育app Corporation, Ashland, MA

Saturday, May 19, 11:45 am – 1:45 pm

Considerable interest exists in evaluating effects on skin pigmentation resulting from treatment with raw materials, skin care formulations, or as a side effect induced by medication.  A convenient way to screen such effects utilizes the MelanoDerm tissue model, a highly differentiated, three-dimensional tissue culture model of human epidermis containing normal human melanocytes and keratinocytes.  使用这种体外模型可以提供有价值的体外数据,作为昂贵的临床试验开始前的早期筛选工具.  In this study, 在2-3周的过程中,使用三刺激色度计来测量黑皮肤组织中正常人类黑素细胞的亮度(L*), Asian, or Caucasian donors.  In parallel to measurements taken with the chromameter, total melanin content of tissues was also quantified.  Over time, 培养物变得越来越色素沉着,并保留正常上皮形态,与预期的色素沉着水平的供体组织, i.e. Black>Asian>Caucasian when cultured in media containing alpha-MSH and beta-FGF.  一些非处方美白产品也在含有来自黑人捐赠者的正常人类黑素细胞的培养中进行了评估. Over the 2-3 week treatment period, 对照培养变得越来越色素沉着,而局部使用含有酪氨酸酶抑制剂(如曲酸和抗坏血酸磷酸镁)的化妆品皮肤美白剂处理的组织与对照培养相比明显更轻.  After 14 days in culture, total melanin content was found to inversely correlate with surface reflectance (L*).  The results described herein suggest that this model is useful for evaluating effects on melanogenesis, skin lightening, and other pigmentation phenomena of skin in vitro.  In particular, this study highlights two distinct endpoints, total melanin content and skin color measurement that can be used to evaluate skin pigmentation in vitro.

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Development of a Do-It-Yourself Skin Equivalent System for Use as a Research Tool | Abstract ID: 431705 Final ID: 715

Michael Bachelor, Kalyani Guntur, Jonathan Oldach and Patrick Hayden

im体育app Corporation, Ashland, MA

Saturday, May 19, 11:45 am – 1:45 pm

Use of 3D skin tissue models can be advantageous over monolayer culture for evaluating basic skin biology, toxicity and efficacy of materials that come in contact with human skin.  Despite the existence of commercially available tissue models, these models may not always be suitable for a specific research focus.  在没有事先专业知识的情况下开发生产皮肤等量物的技术也可能是一项及时和昂贵的工作.  在这里,我们演示了一个三维人体皮肤等效的构建,作为一个自己动手的工具包,用于研究实验室.  All components necessary to build the skin equivalent are provided in the 3DIY kit (im体育app, Ashland, MA), including keratinocytes, pre-coated tissue culture inserts on which to produce the tissue and pre-formulated media.  Our results demonstrate a novice user can successfully produce a 3D skin equivalent using the 3DIY kit that is comparable to a commercially available human skin equivalent (EpiDerm, im体育app Corporation) when using normal neonatal keratinocytes.  Skin equivalents produced with the 3DIY kit versus EpiDerm revealed comparable histology.  As a measure of skin barrier, tissues produced with the 3DIY 试剂盒局部暴露于1% Triton和表皮细胞,发现有相似的ET-50(暴露时间将组织活力降低到50%,用MTT试验确定)。.   The 3DIY 该试剂盒允许更大的实验灵活性,包括(1)角化细胞的遗传操作(通过CRIPSR或RNAi), for example) prior to producing the 3D skin equivalent (2) choosing donor age and sex and (3) donor disease state.   The availability of a ready-to-use technology combined with user flexibility make im体育app’s 3DIY kit a powerful research tool for investigating multiple aspects of skin biology.

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Three Dimensional IHC Characterization of Aging in 3D Skin Models | Abstract ID: 430939 Final ID: 696

M. Johnson1, T. Villani1, M. Bachelor2, J. Oldach2, G. Gardner1

1Visikol Inc, North Brunswick, NJ, and 2im体育app Corporation, Ashland, MA

Thursday, May 17, 11:45 am – 1:45 pm

In the pursuit of reducing animal usage in safety and efficacy studies, there has been a strong focus on developing 3D in vitro cell culture models which closely mimic in vivo tissues. While it has been shown that these 3D in vitro models closely mimic in vivo tissues and do so better than traditional 2D monolayer models, current characterization techniques for these 3D models are limited. Wide field, 用于表征这些模型的共焦或溶解分析,要么失去了使其本质上更有价值的空间信息,要么由于光衰减而仅表征这些模型的外部特征. To address these limitations, 将一种快速、高通量兼容的组织清理技术应用于全厚度人体皮肤3D模型(EpiDermFT),并与荧光免疫标记和高含量共聚焦显微镜相结合. Using inexpensive air objectives and common confocal microscopes,the entire thickness (1 mm) of the 3D skin model could be imaged following tissue clearing. Skin models were successfully labeled for differentiation markers (keratin 14, involucrin), 成纤维细胞特异性标记物(波形蛋白)以及细胞外基质成分(胶原蛋白),结果表明这些标记物可以在模型的整个深度进行表征. The ability to image 3D in vitro 通过整个表皮和真皮层的细胞培养模型,用于共同的衰老相关靶点,将允许开发新的抗衰老特性分析方法.

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