组织学1

Also known generically as a Reconstructed Human Epidermis (RHE), 表皮是现成的, highly differentiated 3D tissue model consisting of normal, 在特制的组织培养衬垫上培养的人源性表皮角化细胞(NHEK).

Insert
组织培养插入

Cultured at the air-liquid interface (ALI), 表皮 allows for the evaluation of topically applied compounds, 化学物质, cosmetic/personal care product ingredients and final formulations. With our straightforward protocols and 20+ years of data, 表皮是一个广泛的高预测的体外应用的金标准.

表皮具有人类表皮组织结构和细胞形态的一致性和可重复性. It’s 3D structure consisting of organized and proliferative basal cells, 棘状和粒状层, and cornified epidermal layers are mitotically and metabolically active.

表皮3D人体组织模型应用范围广泛,包括安全性和风险评估, 和生物有效性. With Air-liquid Interface (ALI) culture technology, 表皮可用于评价制剂或成分局部应用的生物反应. 简单的方案和早期细胞终点的评估允许研究在几天内获得数据, 而不是几周或几个月.

Skin Irritation (OECD TG 439)

Use 表皮 to determine the skin irritation potential of liquids, solids, gels, 乳液, 药膏, 或面霜. Tissue viability results are acquired within 3 days.

Download the Skin Irritation Test Protocol

Skin Corrosion (OECD TG 431)

Determine the skin corrosion potential of test materials in a single day.

Download the Skin Corrosion Protocol

皮肤水合作用

测量表皮的电阻抗,以评估局部应用保湿霜(包括面霜)的效果, gels, 和乳液. The 皮肤水合作用 protocol allows for data analysis within 24 hours.

皮肤水合作用 应用程序 Note

皮肤药

利用现有的透皮渗透设备或im体育app的简单单插入式渗透设备来评估API的渗透和通量. Determine relative safety with the 表皮 MTT ET-50 assay.

Download the Percutaneous 吸收 Protocol

光毒性(OECD TG 498)

使用表皮光毒性试验确定局部应用的测试物质的光毒性效应.

Download the 光毒性 Protocol

使用表皮组织模型确定系统应用测试物质的效果.

Download the Systemic 光毒性 Protocol

真皮基因毒性

使用表皮细胞基因毒性试验(预验证)确定局部或全身试验物质的基因毒性效应

Micronucleus Assay Reference

彗星试验协议

表皮分化

使用不发达的表皮组织模型评估活性化合物对表皮分化的影响

浏览我们的 参考图书馆 to see how our 表皮 tissue has been used in these areas of study.

 

Tissue

Kit: A standard 表皮 kit (EPI-200) consists of 24 tissues. (Tissue “kits” contain tissues, a small amount of culture medium, and plasticware; contact im体育app for specific kit contents)

格式: 9mm & 22mm单个插入和96孔高温高温板-组织培养基质经过化学修饰,孔径为0.4 μm

文化: 气液界面

组织学: 8-12层细胞层加角质层(基底层、棘状层和颗粒层)

很多数字: Tissue lots produced each week are assigned a specific lot number. 在批号的末尾附加一个字母,以区分给定数量组织中的单个试剂盒. All tissue kits within a lot are identical in regards to cells, medium, 处理, 培养条件, etc.

装运: At 4°C on medium-supplemented, agarose gels

装运: 每星期一. Shipment on Thursday also possible upon special request

交付: Tuesday morning via FedEx priority service (US). Outside US: Tuesday-Thursday depending on location

保质期: 包括运输时间,组织在使用前可在4°C保存6天. However, extended storage periods are not recommended unless necessary. 除了, 如果组织在同一天一致使用,可获得最佳的重现性, e.g. 周二下午或4°C过夜后(周三上午)

长度的实验: 可持续培养至少2周,表皮形态保持良好. Cultures must be fed every other day with 5.0 mL of either 新的维护中 (epi - 100 nmm-3) or epi - 100 nmm-113.  细胞培养插入物悬挂顶板(HNG-TOP-12,提供),允许使用5.0 mL. 

Cells

Type: Normal human epidermal keratinocytes (NHEK)

基因组成: 单一捐赠者

来自: Neonatal-foreskin tissue (NHEK)

选择: 来自成人乳房皮肤的NHEK

筛选: HIV, Hepatitis-B, Hepatitis-C, mycoplasma

Medium

基础培养基: Dulbecco’s Modified Eagle’s Medium (DMEM)

生长因子/激素: 表皮生长因子, 胰岛素, 氢化可的松和其他专有的表皮分化刺激剂

Serum: None

抗生素: Gentamicin 5 µg/ml (10% of normal gentamicin level)

抗真菌剂: 两性霉素B 0.25µg / ml

酸碱指示剂: 酚红

其他添加剂: 用于增强表皮屏障形成的脂质前体(专有)

选择: 酚红-free (EPI-200-PRF), antibiotic-free (EPI-200-ABF), anti-fungal-free (EPI-200-AFF), or hydrocortisone-free medium and tissue (EPI-200-HCF) are available. Agents are removed at least 3 days prior to shipment. 请在下单前与im体育app科技人员讨论您的特定介质配方和组织需求.

分析/维护媒介: Three maintenance media are available. 表皮维持培养基(EPI-100-MM)相当于试验培养基(EPI-100-ASY),用于短期毒理学检测(≤24小时). 新的维护中, epi - 100 nmm, 含有角质细胞生长因子(KGF),用于研究长达3天. epi - 100 nmm-3 contains higher levels of KGF and is used for longer-term studies (> 3 days). 为了使表皮形态保持良好至少2周,使用epi - 100 nmm-3 (5.0 mL changed every other day) is recommended.

Quality Control and Sterility

目视检查: 所有组织都要进行肉眼检查,如果发现有物理缺陷, tissues are rejected for shipment

最终用途测试: Tissues are exposed to 1% Triton X-100 for 4, 6, 8 and 12.5 hours. 使用MTT活性测定法测定每批组织降低组织活力(ET-50)所需的暴露时间(见im体育app 表皮 MTT ET-50协议). ET-50’s must fall within the range of the 1996 表皮 database of 4.77 – 8.72小时. ET-50’s in customers’ labs may differ slightly from the im体育app results.

不育: All media used throughout the production process is checked for sterility. 维持培养基加或不加抗生素孵育1周,并检查无菌. 用于运输的24孔板上的琼脂糖凝胶也要培养一周,并检查任何污染的迹象

筛查病原体: 所有的细胞都经过PCR检测,HIV、乙肝和丙肝均呈阴性. 然而,没有一种已知的测试方法可以完全保证细胞是无病原体的. Thus, 这些产品和所有人类衍生产品应按照CDC-NIH手册中建议的BSL-2级别(生物安全级别2)或更高级别处理, “Biosafety in microbiological and biomedical laboratories,” 1998. 如需进一步帮助,请联系您的现场安全主任或im体育app技术服务

Notification of lot failure: If a tissue lot fails our QC or sterility testing, 我们会通知客户,并在适当的时候免费更换纸巾. Because our QC and sterility testing is done post-shipment, notification will be made as soon as possible (Under normal circumstances, ET-50 failures will be notified by Wednesday 5 p.m.; sterility failures will be notified within 8 days of shipment)

请求报价 for an 在体外 Product

申请我们的体外组织产品的报价,我们团队的成员将立即与您联系.
  • 我们需要您的送货地址,包括运费估计为您的货物.

人类的相关性

应用程序